General Laboratory

FACSAria™III- Fluorescence Assisted Cell Sorter (FACS)

Separates or sorts cells that are phenotypically different from each other, as well as, cells that express proteins of interest.
High-speed fixed alignment laser bench top sorter that has the ability to sort or isolate cells of interest from a mixed population of cells.
Equipped with 405 nm, 488 nm, 561 nm, 631 nm lasers and is capable of up to 10 color simultaneous detection
Subpopulations of cells are tagged with a fluorescent-conjugated antibody that binds to a protein uniquely expressed by the cells of interest.
Multiple sorting options, from sorting single population of cells, up to sorting four different cell populations in a temperature-controlled environment.
Single cells may also be sorted directly onto standard cell culture plates.
Sorting, data acquisition, and analysis are controlled by BD FACSDiva software.

Exploits the chiral nature of biological molecules and difference in their interaction with right and left circularly polarized light.
Records circular dichroism spectrum as a function of wavelength, pH, time, temperature and concentration.
Provides excellent quality scans in the far UV range of 195-240 nm at a temperature ranging from 5 °C and 95 °C wavelength, enabling quantitative secondary structure analysis.
Capable of evaluating protein folding events on timescales of 5 ms or longer with the stopped-flow mixing module.
Use 450W Xenon arc lamp with a synthetic fused silica envelope as light source.

A novel high-throughput, plate-based, thermal block cycler with integrated real-time, online detection capabilities.
Provides remarkable well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
Provides the ideal solution for fast and precise qualitative or quantitative detection of genotyping, mutation scanning, and nucleic acids.
Allows the use of all current probe formats (e.g.,SYBR Green I, hydrolysis probes, HybProbe probes, SimpleProbe probes).

Fully automated, programmable instrument that performs the chain assembly steps in solid-phase peptide synthesis (SPPS)
Performs Fmoc Chemistry to carry out SPPS where FastMoc kit (Applied Biosystems). containing HBTU is the activation reagent in the presence of NMP.
Monitor the synthesis by real-time, feedback monitoring feature based on the measurement of conductance by a built in conductivity cell.

A state of the art instrument to determine molecular masses, stoichiometry of oligomerizations, binding constants for associating systems and sample homogeneity through sedimentation velocity and sedimentation equilibrium analyses.
Equipped with sensitive absorbance optics as well as interference optics for sample detection, both detection methods can be simultaneously utilized, if needed.

Real-time imaging of live cells.
Time-lapse imaging.
Imaging of bright field or fluorescently labeled cells.
Calcium imaging.
Environmentally controlled chamber.
Nikon Elements software is used to acquire and process the acquired data. (nikoninstruments.com/Products/Software/NIS-Elements-Ar-Microscope-Imaging-Software)

Fast and easy automated isolation of any cell type from a wide range of sample sources.
Cell types can be isolated using positive or negative selection protocols.
Software is loaded with preprogrammed selection protocols or customized cell separation protocols can be created.
Single aspiration head with 5 mL & 250 µL disposable tips, for zero carryover or cross-contamination risk.
Wide sample dispense range, from 12.5 µL (10% inaccuracy) to 10 mL (0.5 % inaccuracy).
Touch screen graphical user-interface with pictorial text support and user-customizable protocols.
Automated magnetic particle separation.
Standard bio-containment hood for sterile operation.

Fully integrated and motorized patch clamping rig for both in vitro, in vivo and ex vivo studies.
Customized build for slice electrophysiology, florescent imaging, and patch clamp electrophysiology.
Includes LinLab software, allowing customizable control of the micromanipulators.
Provides less than 1 micron drift in 2hours for reliable long term experiments.
Slices prepared using the Leica VT1200S Vibratome.
Borosilicate micropipettes pulled to 3-5 MΩ resistance using Sutter Instruments P-97 Micropipette Puller.

If your research would benefit from the use of any W.M. Keck Center instrumentation, please contact Victoria Ploplis for availability.