General Laboratory

FACSAria™III- Fluorescence Assisted Cell Sorter (FACS)

FACSAria™III- Fluorescence Assisted Cell Sorter (FACS)

www.bdbiosciences.com/instruments/facsaria/

  • Separates or sorts cells that are phenotypically different from each other, as well as, cells that express proteins of interest
  • High-speed fixed alignment laser bench top sorter that has the ability to sort or isolate cells of interest from a mixed population of cells. 
  • Equipped with 405 nm, 488 nm, 561 nm, 631 nm lasers and is capable of up to 10 color simultaneous detection
  • Subpopulations of cells are tagged with a fluorescent-conjugated antibody that binds to a protein uniquely expressed by the cells of interest
  • Multiple sorting options, from sorting single population of cells, up to sorting four different cell populations in a temperature-controlled environment
  • Single cells may also be sorted directly onto standard cell culture plates 
  • Sorting, data acquisition, and analysis are controlled by BD FACSDiva software.

AVIV Stopped-Flow Circular Dichroism 202SF Spectrometer

AVIV Stopped-Flow Circular Dichroism 202SF Spectrometer

http://www.avivbiomedical.com/

  • Exploits the chiral nature of biological molecules and difference in their interaction with right and left circularly polarized light
  • Records circular dichroism spectrum as a function of wavelength, pH, time, temperature and concentration
  • Provides excellent quality scans in the far UV range of 195-240 nm at a temperature ranging from 5 °C and 95 °C wavelength, enabling quantitative secondary structure analysis
  • Capable of evaluating protein folding events on timescales of 5 ms or longer with the stopped-flow mixing module
  • Use 450W Xenon arc lamp with a synthetic fused silica envelope as light source


LightCycler® 480 Real-Time PCR System

LightCycler® 480 Real-Time PCR System

http://www.roche.com/products/product-details.htm?region=us&type=product&id=64

  • A novel high-throughput, plate-based, thermal block cycler with integrated real-time, online detection capabilities
  • Provides remarkable well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility
  • Provides the ideal solution for fast and precise qualitative or quantitative detection of genotyping, mutation scanning, and nucleic acids
  • Allows the use of all current probe formats (e.g.,SYBR Green I, hydrolysis probes, HybProbe probes, SimpleProbe probes)

ABI 433A Peptide Synthesizer

ABI 433A Peptide Synthesizer

http://tools.lifetechnologies.com/content/sfs/manuals/cms_041851.pdf

  • Fully automated, programmable instrument that performs the chain assembly steps in solid phase peptide synthesis (SPPS)
  • Performs Fmoc Chemistry to carry out SPPS where FastMoc kit (Applied Biosystems) containing HBTU is the activation reagent in the presence of NMP
  • Monitor the synthesis by real-time, feedback monitoring feature based on the measurement of conductance by a built in conductivity cell

Optima XLI Analytical Ultracentrifuge

Optima XLI Analytical Ultracentrifuge

https://www.beckmancoulter.com/wsrportal/techdocs?docname=LXLAI-IM-10

  • A state of the art instrument to determine molecular masses, stoichiometry of oligomerizations, binding constants for associating systems and sample homogeneity through sedimentation velocity and sedimentation equilibrium analyses
  • Equipped with sensitive absorbance optics as well as interference optics for sample detection, both detection methods can be simultaneously utilized if needed

Real-Time Imaging Microscopy

Real-Time Imaging Microscopy

http://www.nikon-instruments.jp/eng/page/products/te2000.aspx

  • Real-time imaging of live cells
  • Time-lapse imaging
  • Imaging of bright field or fluorescently labeled cells
  • Calcium imaging
  • Environmentally controlled chamber
  • Nikon Elements software is used to acquire and process the acquired data
  • nikoninstruments.com/Products/Software/NIS-Elements-Ar-Microscope-Imaging-Software

RoboSep™- Fully Automated Magnetic Cell Separator

RoboSep™- Fully Automated Magnetic Cell Separator

http://www.stemcell.com/

  • Fast and easy automated isolation of any cell type from a wide range of sample sources
  • Cell types can be isolated using positive or negative selection protocols
  • Software is loaded with preprogrammed selection protocols or customized cell separation protocols can be created
  • Single aspiration head with 5 mL & 250 µL disposable tips, for zero carryover or cross contamination risk
  • Wide sample dispense range, from 12.5 µL (10% inaccuracy) to 10 mL (0.5 % inaccuracy)
  • Touch screen graphical user-interface with pictorial text support and user customizable protocols
  • Automated magnetic particle separation
  • Standard bio-containment hood for sterile operation

SliceScope Pro 6000 Electrophysiology Rig

SliceScope Pro 6000 Electrophysiology Rig

http://www.scientifica.uk.com/products/slicescope-pro-6000

  • Fully integrated and motorized patch clamping rig for both in vitro, in vivo and ex vivo studies
  • Customized build for slice electrophysiology, florescent imaging, and patch clamp electrophysiology
  • Includes LinLab software, allowing customizable control of the micromanipulators
  • Provides less than 1 micron drift in 2hours for reliable long term experiments
  • Slices prepared using the Leica VT1200S Vibratome
  • Borosilicate micropipettes pulled to 3-5 MΩ resistance using Sutter Instruments P-97 Micropipette Puller