Zhong Liang, MS

Senior Scientist, Department of Chemistry and Biochemistry

Zhong -  Liang, MS

Contact Info

Phone: 574-631-2320
Email: Zhong.Liang.16@nd.edu

Biography

2014–present Senior Scientist, Department of Chemistry and Biochemistry, Harper Cancer Research Institute, University of Notre Dame
2008–2014 Associate Professional Specialist, W.M.Keck Center for Transgene Research, University of Notre Dame
2004–2008 Assistant Scientist, Medical and Molecular Genetics, School of Medicine,Indiana University
1998–2004 Assistant Professional Specialist, W.M. Keck Center for Transgene Research, University of Notre Dame
1997–1998 Fellow, Department of Chemistry and Biochemistry, University of Notre Dame
1994–1997 Graduate student (Ph. D. program, Supervisors: Prof. D. Collen and Prof. P. Carmeliet) Center for Transgene Technology and Gene Therapy, Leuven University, Leuven, Belgium
1994 Research Assistant, Blood Transfusion Center, Leuven, Belgium
1993–1994 Research Assistant, Molecular Biology for Mycobacteria, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium
1991–1993 Graduate student (M. Sc. program, Supervisor: Prof. V. Kumar), Institute of Tropical Medicine, Antwerp, Belgium
1984–1991 Research Assistant, Marine Biology and Parasitology,Tianjin Natural History Museum, Tianjin, PR China
1985–1991 Research Fellow,Taxology and Ecology of Trematodes,Nankai University, Tianjin, PR China
1989–1990 Research Fellow,The Function of Zoning of China–Sea (mainly marine biology),State Oceanic Administration, Beijing, PR China
1982–1988 Research Fellow, The Investigation of Coastal Resources in China, Tianjin, PR China
1982–1984 Teaching, Marine Biology, Tianjin Fishery School, Tianjin, PR China
1978–1982 Undergraduate student, B.Sc., China Ocean University, Qingdao, PR China

Honors & Awards 

1994–1997

Leuven Univ. Scholarship
1993–1994 Belgium Red–Cross Fellowship
1989 China National Prize (second/group)
1989 China National Prize (second/personal)
1982–1989 China National Fund (three items)
1986–1988 State Fund (two items)
1977–1982  National Scholarship

Research Interest

Molecular Biology, gene squencing

Recent Papers

Glinton K, Beck J, Liang Z, Qiu C, Lee SW, Ploplis VA, Castellino FJ. Variable Region in Streptococcal M-Proteins Provides Stable Binding with Host Fibrinogen for Plasminogen-Mediated Bacterial Invasion. J Biol Chem. 2017 Mar 9. pii: jbc.M116.768937. doi: 10.1074/jbc.M116.768937. [Epub ahead of print] PMID: 28280245

Bao YJ, Liang Z, Mayfield JA, McShan WM, Lee SW, Ploplis VA, Castellino FJ. Novel genomic rearrangements mediated by multiple genetic elements in Streptococcus pyogenes M23ND confer potential for evolutionary persistence. Microbiology. 2016 Aug;162(8):1346-59. doi: 10.1099/mic.0.000326. Epub 2016 Jun 21. PMID: 27329479

Bao YJ, Liang Z, Mayfield JA, Donahue DL, Carothers KE, Lee SW, Ploplis VA, Castellino FJ. Genomic Characterization of a Pattern D Streptococcus pyogenes emm53 Isolate Reveals a Genetic Rationale for Invasive Skin Tropicity. J Bacteriol. 2016 May 27;198(12):1712-24. doi: 10.1128/JB.01019-15. Print 2016 Jun 15. PMID: 27044623

Agrahari G, Liang Z, Glinton K, Lee SW, Ploplis VA, Castellino FJ. Streptococcus pyogenes Employs Strain-dependent Mechanisms of C3b Inactivation to Inhibit Phagocytosis and Killing of Bacteria. J Biol Chem. 2016 Apr 22;291(17):9181-9. doi: 10.1074/jbc.M115.704221. Epub 2016 Mar 4. PMID: 26945067

Bao YJ, Liang Z, Mayfield JA, Lee SW, Ploplis VA, Castellino FJ. CovRS-Regulated Transcriptome Analysis of a Hypervirulent M23 Strain of Group A Streptococcus pyogenes Provides New Insights into Virulence Determinants. J Bacteriol. 2015 Oct;197(19):3191-205. doi: 10.1128/JB.00511-15. Epub 2015 Jul 27. PMID: 26216843

Chandrahas V, Glinton K, Liang Z, Donahue DL, Ploplis VA, Castellino FJ. Direct Host Plasminogen Binding to Bacterial Surface M-protein in Pattern D Strains of Streptococcus pyogenes Is Required for Activation by Its Natural Coinherited SK2b Protein. J Biol Chem. 2015 Jul 24;290(30):18833-42. doi: 10.1074/jbc.M115.655365. Epub 2015 Jun 12. PMID: 26070561

Bao Y, Liang Z, Booyjzsen C, Mayfield JA, Li Y, Lee SW, Ploplis VA, Song H, Castellino FJ. Unique genomic arrangements in an invasive serotype M23 strain of Streptococcus pyogenes identify genes that induce hypervirulence. J Bacteriol. 2014 Dec;196(23):4089-102. doi: 10.1128/JB.02131-14. Epub 2014 Sep 15. PMID: 25225265

Mayfield JA, Liang Z, Agrahari G, Lee SW, Donahue DL, Ploplis VA, Castellino FJ. Mutations in the control of virulence sensor gene from Streptococcus pyogenes after infection in mice lead to clonal bacterial variants with altered gene regulatory activity and virulence. PLoS One. 2014 Jun 26;9(6):e100698. doi: 10.1371/journal.pone.0100698. eCollection 2014. PMID:24968349

Bhattacharya S, Liang Z, Quek AJ, Ploplis VA, Law R, Castellino FJ. Dimerization Is Not a Determining Factor for Functional High Affinity Human Plasminogen Binding by the Group A Streptococcal Virulence Factor PAM and Is Mediated by Specific Residues within the PAM a1a2 Domain. J Biol Chem. 2014 Aug 1;289(31):21684-93. doi: 10.1074/jbc.M114.570218. Epub 2014 Jun 24. PMID:24962580

Agrahari G, Liang Z, Mayfield JA, Balsara RD, Ploplis VA, Castellino FJ. Complement-mediated opsonization of invasive group A Streptococcus pyogenes strain AP53 is regulated by the bacterial two-component cluster of virulence responder/sensor (CovRS) system. J Biol Chem. 2013 Sep 20;288(38):27494-504. doi: 10.1074/jbc.M113.494864. Epub 2013 Aug 8. PMID:23928307

Zhang Y, Liang Z, Glinton K, Ploplis VA, Castellino FJ. Functional differences between Streptococcus pyogenes cluster 1 and cluster 2b streptokinases are determined by their β-domains. FEBS Lett. 2013 May 2;587(9):1304-9. doi: 10.1016/j.febslet.2013.02.033. Epub 2013 Mar 7. PMID:23474243

Liang Z, Zhang Y, Agrahari G, Chandrahas V, Glinton K, Donahue DL, Balsara RD, Ploplis VA, Castellino FJ. A natural inactivating mutation in the CovS component of the CovRS regulatory operon in a pattern D Streptococcal pyogenes strain influences virulence-associated genes. J Biol Chem. 2013 Mar 1;288(9):6561-73. doi: 10.1074/jbc.M112.442657. Epub 2013 Jan 13. PMID:23316057