Deborah Donahue, BS

Associate Professional Specialist

Deborah - Donahue, BS

Contact Info

Phone: 574-631-2318
Email: ddonahu1@nd.edu

Biography

2007–present Associate Professional Specialist, W. M. Keck Center for Transgene Research, University of Notre Dame
2001–2007 Assistant Professional Specialist, W. M. Keck Center for Transgene Research, University of Notre Dame
1996–2001 Surgical Specialist, Surgical Department, Taconic Farms, Inc.
1996 B.S., Cornell University
1994 A.S., Columbia–Greene Community College

Honors & Awards

1999 Laboratory Animal Technician, AALAS certification

Research Interests

My interests include the use of rodent models to mimic human disease states and to determine the extent of these diseases as a function of altered gene expression. The animal models developed may be genetically predisposed and/or mechanically induced. Some of the types of models that have been developed consist of a rat model of traumatic brain injury, murine models of spontaneous and induced cardiac fibrosis, ovarian transplants, and a middle cerebral artery occlusion model of stroke. Imaging and the use of non-invasive measurements have been used in conjunction with these models to reduce the number of animals needed for each study. The Center has use of an ultrasound, endoscope, x-ray irradiator, telemetry, and various blood assessment instruments.Research Interests

The instrument that has been a recent main focus is the thromboelastograph (TEG) which measures whole blood clotting. This instrument has been used in collaboration with Memorial Hospital in assessing traumatic brain injury, sepsis, and studying the coagulation profiles of people with platelet dysfunction. These studies have linked the observed basic science as well as clinically changing point-of–care for patients.

Recent Papers

Gupta KK, Donahue DL, Sandoval-Cooper MJ, Castellino FJ, Ploplis VA. Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation. Sci Rep. 2017 Mar 23;7(1):365. doi: 10.1038/s41598-017-00418-y. PMID: 28336948

Higashi DL, Biais N, Donahue DL, Mayfield JA, Tessier CR, Rodriguez K, Ashfeld BL, Luchetti J, Ploplis VA, Castellino FJ, Lee SW. Activation of band 3 mediates group A Streptococcus streptolysin S-based beta-haemolysis. Nat Microbiol. 2016 Jan 18;1:15004. doi: 10.1038/nmicrobiol.2015.4. PMID: 27571972

Yasui H, Donahue DL, Walsh M, Castellino FJ, Ploplis VA. Early coagulation events induce acute lung injury in a rat model of blunt traumatic brain injury. Am J Physiol Lung Cell Mol Physiol. 2016 Jul 1;311(1):L74-86. doi: 10.1152/ajplung.00429.2015. Epub 2016 May 17. PMID: 27190065

Bao YJ, Liang Z, Mayfield JA, Donahue DL, Carothers KE, Lee SW, Ploplis VA, Castellino FJ. Genomic Characterization of a Pattern D Streptococcus pyogenes emm53 Isolate Reveals a Genetic Rationale for Invasive Skin Tropicity. J Bacteriol. 2016 May 27;198(12):1712-24. doi: 10.1128/JB.01019-15. Print 2016 Jun 15. PMID: 27044623

Chandrahas V, Glinton K, Liang Z, Donahue DL, Ploplis VA, Castellino FJ. Direct Host Plasminogen Binding to Bacterial Surface M-protein in Pattern D Strains of Streptococcus pyogenes Is Required for Activation by Its Natural Coinherited SK2b Protein. J Biol Chem. 2015 Jul 24;290(30):18833-42. doi: 10.1074/jbc.M115.655365. Epub 2015 Jun 12. PMID: 26070561

Balsara R, Dang A, Donahue DL, Snow T, Castellino FJ. Conantokin-G attenuates detrimental effects of NMDAR hyperactivity in an ischemic rat model of stroke. PLoS One. 2015 Mar 30;10(3):e0122840. doi: 10.1371/journal.pone.0122840. eCollection 2015 Mar 30. PMID: 25822337

Gupta KK, Donahue DL, Sandoval-Cooper MJ, Castellino FJ, Ploplis VA. Abrogation of plasminogen activator inhibitor-1-vitronectin interaction ameliorates acute kidney injury in murine endotoxemia. PLoS One. 2015 Mar 23;10(3):e0120728. doi: 10.1371/journal.pone.0120728. eCollection 2015 Mar 23. PMID: 25799354

Balsara RD, Chapman SE, Sander IM, Donahue DL, Liepert L, Castellino FJ, Leevy WM. Non-invasive imaging and analysis of cerebral ischemia in living rats using positron emission tomography with 18F-FDG. J Vis Exp. 2014 Dec 28;(94). doi: 10.3791/51495. PMID: 25590998

Mayfield JA, Liang Z, Agrahari G, Lee SW, Donahue DL, Ploplis VA, Castellino FJ. Mutations in the control of virulence sensor gene from Streptococcus pyogenes after Infection in mice lead to clonal bacterial variants with altered gene regulatory activity and virulence. PLoS One. 2014 Jun 26;9(6):e100698. doi: 10.1371/journal.pone.0100698. eCollection 2014. PMID:24968349

Castellino FJ, Chapman MP, Donahue DL, Thomas S, Moore EE, Wohlauer MV, Fritz B, Yount R, Ploplis V, Davis P, Evans E, Walsh M. Traumatic brain injury causes platelet adenosine diphosphate and arachidonic acid receptor inhibition independent of hemorrhagic shock in humans and rats. J Trauma Acute Care Surg. 2014 May;76(5):1169-76. doi: 10.1097/TA.0000000000000216. PMID:24747445

Ploplis VA, Donahue DL, Sandoval-Cooper MJ, MorenoCaffaro M, Sheets P, Thomas SG, Walsh M, Castellino FJ. Systemic Platelet Dysfunction Is the Result of Local Dysregulated Coagulation and Platelet Activation in the Brain in a Rat Model of Isolated Traumatic Brain Injury. J Neurotrauma. 2014 May 21. PMID:24605991

Balsara RD, Ferreira AN, Donahue DL, Castellino FJ, Sheets PL. Probing NMDA receptor GluN2A and GluN2B subunit expression and distribution in cortical neurons. Neuropharmacology. 2014 Apr;79:542-9. doi: 10.1016/j.neuropharm.2014.01.005. Epub 2014 Jan 15. PMID:24440368

Donahue DL, Beck J, Fritz B, Davis P, Sandoval-Cooper MJ, Thomas SG, Yount RA, Walsh M, Ploplis VA, Castellino FJ. Early platelet dysfunction in a rodent model of blunt traumatic brain injury reflects the acute traumatic coagulopathy found in humans. J Neurotrauma. 2014 Feb 15;31(4):404-10. doi: 10.1089/neu.2013.3089. Epub 2013 Nov 21. PMID:24040968